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P62/SQSTM1 antibody
貨 號：KCD92734
產品規格：100ul
價 格：￥2800
周 期：
產品介紹
| 經測試應用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| 文獻引用應用 | WB, IHC, IF, IP, CoIP |
| 經測試反應性 | Human |
經過測試的應用
| Positive WB detected in | HeLa cells, U2OS cells, HEK-293 cells, HepG2 cells, U-87 MG cells, Jurkat cells |
| Positive IP detected in | HepG2 cells, U2OS cells |
| Positive IHC detected in | human lung cancer tissue, human gliomas tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Chloroquine treated HepG2 cells, U2OS cells, Chloroquine treated HeLa cells |
| Positive FC (Intra) detected in | HEK-293 cells |
推薦稀釋比
| 應用 | 推薦稀釋比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 μl suspension |
