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Alpha Tubulin antibody
貨 號：KCD73749
產品規格：100ul
價 格：￥2800
周 期：
產品介紹
| 經測試應用 | WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA |
| 文獻引用應用 | WB, IHC, IF, IP, CoIP |
| 經測試反應性 | Human, Mouse, Rat |
經過測試的應用
| Positive WB detected in | HEK-293 cells, A549 cells, HeLa cells, NIH/3T3 cells, rat brain, C6 cells, mouse heart tissue, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human liver cancer tissue, human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-Fro detected in | mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells, MCF-7 cells, Lymphatic endothelial cells cells |
| Positive FC (Intra) detected in | HEK-293 cells |
推薦稀釋比
| 應用 | 推薦稀釋比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 μl suspension |
