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HIF-1 alpha antibody
貨 號：kCD32675
產品規格：100ul
價 格：￥2800
周 期：
產品介紹
| 經測試應用 | WB, IHC, IF/ICC, IP, ELISA |
| 文獻引用應用 | WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA |
| 經測試反應性 | Human |
經過測試的應用
| Positive WB detected in | Cobalt Chloride treated HeLa cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human thyroid cancer tissue, human heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Cobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells |
推薦稀釋比
| 應用 | 推薦稀釋比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
